Predicting functional responses in agro-ecosystems from animal movement data to improve management of invasive pests

Functional responses describe how changing resource availability affects con- sumer resource use, thus providing a mechanistic approach to prediction of the invasibility and potential damage of invasive alien species (IAS). However, functional responses can be context dependent, varying with resource characteristics and availability, consumer attributes, and environmental variables. Identifying context dependencies can allow invasion and damage risk to be predicted across different ecoregions. Understanding how ecological factors shape the functional response in agro-ecosystems can improve predictions of hotspots of highest impact and inform strategies to mitigate damage across locations with varying crop types and avail- ability. We linked heterogeneous movement data across different agro-ecosystems to predict ecologically driven variability in the functional responses. We applied our approach to wild pigs (Sus scrofa), one of the most successful and detrimental IAS worldwide where agricultural resource depredation is an important driver of spread and establishment. We used continental- scale movement data within agro-ecosystems to quantify the functional response of agricul- tural resources relative to availability of crops and natural forage. We hypothesized that wild pigs would selectively use crops more often when natural forage resources were low. We also examined how individual attributes such as sex, crop type, and resource stimulus such as dis- tance to crops altered the magnitude of the functional response. There was a strong agricul- tural functional response where crop use was an accelerating function of crop availability at low density (Type III) and was highly context dependent. As hypothesized, there was a reduced response of crop use with increasing crop availability when non-agricultural resources were more available, emphasizing that crop damage levels are likely to be highly heterogeneous depending on surrounding natural resources and temporal availability of crops. We found sig- nificant effects of crop type and sex, with males spending 20% more time and visiting crops 58% more often than females, and both sexes showing different functional responses depend- ing on crop type. Our application demonstrates how commonly collected animal movement data can be used to understand context dependencies in resource use to improve our under- standing of pest foraging behavior, with implications for prioritizing spatiotemporal hotspots of potential economic loss in agro-ecosystems

The Observed Evolution of the Stellar Mass-Halo Mass Relation for Brightest Central Galaxies

We quantify evolution in the cluster-scale stellar mass–halo mass (SMHM) relation's parameters using 2323 clusters and brightest central galaxies (BCGs) over the redshift range 0.03 ≤ z ≤ 0.60. The precision on the inferred SMHM parameters is improved by including the magnitude gap (mgap) between the BCG and fourth-brightest cluster member (M14) as a third parameter in the SMHM relation. At fixed halo mass, accounting for mgap, through a stretch parameter, reduces the SMHM relation's intrinsic scatter. To explore this redshift range, we use clusters, BCGs, and cluster members identified using the Sloan Digital Sky Survey C4 and redMaPPer cluster catalogs and the Dark Energy Survey redMaPPer catalog. Through this joint analysis, we detect no systematic differences in BCG stellar mass, mgap, and cluster mass (inferred from richness) between the data sets. We utilize the Pareto function to quantify each parameter's evolution. We confirm prior findings of negative evolution in the SMHM relation's slope (3.5σ), and detect negative evolution in the stretch parameter (4.0σ) and positive evolution in the offset parameter (5.8σ). This observed evolution, combined with the absence of BCG growth, when stellar mass is measured within 50 kpc, suggests that this evolution results from changes in the cluster's mgap. For this to occur, late-term growth must be in the intracluster light surrounding the BCG. We also compare the observed results to IllustrisTNG 300-1 cosmological hydrodynamic simulations and find modest qualitative agreement. However, the simulations lack the evolutionary features detected in the real data

Impact of COVID-19 on cardiovascular testing in the United States versus the rest of the world

Objectives: This study sought to quantify and compare the decline in volumes of cardiovascular procedures between the United States and non-US institutions during the early phase of the coronavirus disease-2019 (COVID-19) pandemic. Background: The COVID-19 pandemic has disrupted the care of many non-COVID-19 illnesses. Reductions in diagnostic cardiovascular testing around the world have led to concerns over the implications of reduced testing for cardiovascular disease (CVD) morbidity and mortality. Methods: Data were submitted to the INCAPS-COVID (International Atomic Energy Agency Non-Invasive Cardiology Protocols Study of COVID-19), a multinational registry comprising 909 institutions in 108 countries (including 155 facilities in 40 U.S. states), assessing the impact of the COVID-19 pandemic on volumes of diagnostic cardiovascular procedures. Data were obtained for April 2020 and compared with volumes of baseline procedures from March 2019. We compared laboratory characteristics, practices, and procedure volumes between U.S. and non-U.S. facilities and between U.S. geographic regions and identified factors associated with volume reduction in the United States. Results: Reductions in the volumes of procedures in the United States were similar to those in non-U.S. facilities (68% vs. 63%, respectively; p = 0.237), although U.S. facilities reported greater reductions in invasive coronary angiography (69% vs. 53%, respectively; p < 0.001). Significantly more U.S. facilities reported increased use of telehealth and patient screening measures than non-U.S. facilities, such as temperature checks, symptom screenings, and COVID-19 testing. Reductions in volumes of procedures differed between U.S. regions, with larger declines observed in the Northeast (76%) and Midwest (74%) than in the South (62%) and West (44%). Prevalence of COVID-19, staff redeployments, outpatient centers, and urban centers were associated with greater reductions in volume in U.S. facilities in a multivariable analysis. Conclusions: We observed marked reductions in U.S. cardiovascular testing in the early phase of the pandemic and significant variability between U.S. regions. The association between reductions of volumes and COVID-19 prevalence in the United States highlighted the need for proactive efforts to maintain access to cardiovascular testing in areas most affected by outbreaks of COVID-19 infection

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals &lt;1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Antibodies against endogenous retroviruses promote lung cancer immunotherapy

B cells are frequently found in the margins of solid tumours as organized follicles in ectopic lymphoid organs called tertiary lymphoid structures (TLS). Although TLS have been found to correlate with improved patient survival and response to immune checkpoint blockade (ICB), the underlying mechanisms of this association remain elusive. Here we investigate lung-resident B cell responses in patients from the TRACERx 421 (Tracking Non-Small-Cell Lung Cancer Evolution Through Therapy) and other lung cancer cohorts, and in a recently established immunogenic mouse model for lung adenocarcinoma. We find that both human and mouse lung adenocarcinomas elicit local germinal centre responses and tumour-binding antibodies, and further identify endogenous retrovirus (ERV) envelope glycoproteins as a dominant anti-tumour antibody target. ERV-targeting B cell responses are amplified by ICB in both humans and mice, and by targeted inhibition of KRAS(G12C) in the mouse model. ERV-reactive antibodies exert anti-tumour activity that extends survival in the mouse model, and ERV expression predicts the outcome of ICB in human lung adenocarcinoma. Finally, we find that effective immunotherapy in the mouse model requires CXCL13-dependent TLS formation. Conversely, therapeutic CXCL13 treatment potentiates anti-tumour immunity and synergizes with ICB. Our findings provide a possible mechanistic basis for the association of TLS with immunotherapy response

Biodegradation of vinyl sulfone reactive dyes

M.S.Raymond K. Fleg

EVALUATING THE IMPACTS OF BLACK-TAILED PRAIRIE DOGS ON VEGETATION IN TRADITIONAL AND NON-TRADITIONAL HABITATS

The wildland-urban interface (WUI), defined as areas where human development meets undeveloped wildland (Radeloff et al. 2005), is a focal area for humanwildlife interactions in many communities of the western United States, particularly in those areas that have experienced rapid and expansive human population growth. Since 1960, conversion of rural to urban land has more than doubled in the United States (Theobald 2001). The eastern front range of the Rocky Mountains has experienced one of the most rapid urban expansions in the country, with approximately 110,000 hectares of undeveloped rural land being converted to human-developed land every year between 1992 and 1997 (Obermann et al. 2000, Maestas et al. 2001). In grassland remnants within the WUI, many native wildlife species, including black-tailed prairie dogs (Cynomys ludovicanus), persist and land managers are faced with decisions about how to manage these wildlife population

Radiation oncologists\u27 response to pundendal entrapment neuropathy

Comment on: Pudendal entrapment neuropathy: a rare complication of pelvic radiation therapy. [Pain Physician. 2013

EVALUATING THE IMPACTS OF BLACK-TAILED PRAIRIE DOGS ON VEGETATION IN TRADITIONAL AND NON-TRADITIONAL HABITATS

The wildland-urban interface (WUI), defined as areas where human development meets undeveloped wildland (Radeloff et al. 2005), is a focal area for humanwildlife interactions in many communities of the western United States, particularly in those areas that have experienced rapid and expansive human population growth. Since 1960, conversion of rural to urban land has more than doubled in the United States (Theobald 2001). The eastern front range of the Rocky Mountains has experienced one of the most rapid urban expansions in the country, with approximately 110,000 hectares of undeveloped rural land being converted to human-developed land every year between 1992 and 1997 (Obermann et al. 2000, Maestas et al. 2001). In grassland remnants within the WUI, many native wildlife species, including black-tailed prairie dogs (Cynomys ludovicanus), persist and land managers are faced with decisions about how to manage these wildlife population

Detection and prevalence of Rosellinia necatrix in South African avocado orchards

Rosellinia necatrix is an ascomycete that causes white root rot (WRR) of several plant host species resulting in economic losses to affected agricultural and forestry industries in various regions. This study aimed to identify and monitor the prevalence of R. necatrix in avocado orchards in South Africa. We used both morphological and molecular methods to isolate and identify R. necatrix from diseased plant material and soil. Results showed that R. necatrix was present on avocado in the Limpopo, Mpumalanga and KwaZulu-Natal provinces. Additionally, a semi-selective medium, containing Rose Bengal, nystatin, cycloheximide, chlorothalonil and 2-phenylphenol, was developed to improve isolation of R. necatrix. We also tested an already established R. necatrix-specific TaqMan qPCR protocol to determine if it can reliably detect the pathogen isolates in planta in the South African samples. Based on our results the technique had a detection rate of 91.3% in artificially infected roots and 100% in artificially inoculated soil. We tested natural infected plant and soil samples and detected R. necatrix in 86% of the plant samples and in 70% of the soil samples. Using a selective medium or an in planta molecular detection method streamlines isolation and detection of R. necatrix, which will help prevent further spread of the pathogen. Moreover, additional information on the prevalence of WRR will create awareness among growers and provide a basis for management of the disease.Hans Merensky Foundation and the South African Avocado Growers’ Association.https://link.springer.com/journal/10658hj2022BiochemistryForestry and Agricultural Biotechnology Institute (FABI)GeneticsMicrobiology and Plant Patholog